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The lysis buffer was prepared by mixing 10 ml of M-PER (ThermoFisher) with 100 l of Protease & Phosphatase inhibitor mix (ThermoScientific) and 100 l of 0.5 M EDTA (ThermoScientific)
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Cycling serves to: Prevent GHRH receptor desensitisation or downregulation Maintain the pituitary's sensitivity to endogenous GH-releasing signals Preserve normal hypothalamic-pituitary feedback mechanisms Summary CJC-1295 and Ipamorelin represent two complementary approaches to stimulating growth hormone release
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